Coding
Hfq

Part:BBa_K5108000:Design

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)


RNA binding protein Hfq from Pseudomonas fluorescens ATCC13525


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All BioBrick parts used for the are compatible with the RFC1000 Biobrick standard. Fusion of basic parts was performed following In-Fusion Assembly (Takara, France).

Source

Synthetized from the genomic sequence of Pseudomonas fluorescens ATCC13525, codon optimized for Pseudomonas putida with Integrated DNA Technologies codon optimizer tool. This step permits to lower complexity and minimize secondary structures.

References

  • UniProt. (s. d.-c). https://www.uniprot.org/uniprotkb/C3KDW3/entry
  • Trouillon J, Han K, Attrée I & Lory S (2022) The core and accessory Hfq interactomes across Pseudomonas aeruginosa lineages. Nat Commun 13: 1258
  • Wu P, Wang Z, Zhu Q, Xie Z, Mei Y, Liang Y & Chen Z (2021) Stress preadaptation and overexpression of rpoS and hfq genes increase stress resistance of Pseudomonas fluorescens ATCC13525. Microbiological Research 250: 126804
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